Study of Genetic Diversity of Native Indonesian Catfish Based on Nucleotide Sequences of the ND1 Gene

One of the native catfish to Indonesia is baung fish (Hemibagrus). Baung are found in rivers Sumatra, Kalimantan and Java. The population declining, thus conservation needed prevent extinction. To conduct an effective efforts, molecular studies confirm species from three islands. Mitochondrial DNA one markers that often used see lineage kinship animals for purposes. purpose this study determine genetic diversity NADH Dehydrogenase Subunit I (ND1) gene each these locations, can be as a marker. Samples were obtained their natural habitat, namely Magelang (5), Palembang (3), Riau (2), Samarinda 2 Sintang, 3 Banjarmasin. sample was then isolated template amplification fragments using PCR techniques. Amplikon (PCR product) purified by gel extraction sequenced sequence. potential sequences proven analyzing between MEGA version 7.0 program (Kumar et al., 2016). sequencing results 972 nucleotides composing ND gene, differences 268 nucleotide sites 47 amino acid sites. Based on sequence ND1 Progo river (Magelang, Central Java), Musi (Palembang, Sumatra), Kampar (Riau), Kapuas (Sintang, Kalimantan), Martapura (Banjarmasin), Mahakam (Kalimantan) included Hemibagrus sp.; Elo Java) belongs genus Mystus Bengawan Solo (Bojonegoro, East Pangasius sp.